Mouse brain xsection7/4/2023 ![]() ![]() Zbtb20 implements these temporal effects in part by binding to promoter of the orphan nuclear receptor CoupTF1/Nr2f1. This defect was due to a temporal misbalance in the production of earlier versus later born neurons, leading to a progressive diminishing of the progenitor pool for the generation of 元-L2 neurons. Zbtb20 knock out mice exhibited enhanced populations of early born L6-L4 neuronal subtypes and a dramatic reduction of the late born 元/L2 neurons. Here, we report that during embyogenesis transcription factor Zbtb20 has a dynamic spatio-temporal expression pattern in mitotic cortical progenitors through which it modulates the sequential generation of cortical neuronal layer identities. Transcription factor Zbtb20 has been shown to play a role for hippocampal development but whether it is implicated in mammalian neocortical morphogenesis remains unknown. 10.1002/ corticogenesis, genetic programs encoded in progenitor cells at different developmental stages and inherited in postmitotic neurons specify distinct layer and area identities. Rodent models of depression: forced swim and tail suspension behavioral despair tests in rats and mice. Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull-meninges connections. Principal warps: thin-plate splines and the decomposition of deformations. ![]() Transcriptomic and morphophysiological evidence for a specialized human cortical GABAergic cell type. Whole brain imaging with serial two-photon tomography. The scale bars are for (C) 2 mm and for (E) 200 μm.Īmato S. (E) c-Fos activation was significantly reduced in the PAG, PVT, VMH, and LS in aging mice. Scatter points represent the c-Fos activation of each animal. For all panels, n = 4 per group, * indicates false discovery group (FDR) q-value < 0.05, ** indicates FDR q-value < 0.01, based on P-values from unpaired two-sample t-test comparing the young group to the aging group. (D) The analysis of c-Fos activation in multiple subcortical brain areas. (C) A large number of neuron signals appeared in many cortical and subcortical areas. For all panels, n = 9 per group, * indicates false discovery group (FDR) q-value < 0.05, based on P-values from unpaired two-sample t-test comparing the young group to the aging group. (B) The aging mice showed a significant reduction in the number and time of climbing in the FST. (A) Three behavior postures of the mouse in FST, namely, immobility, swimming, and climbing. High-throughput mouse brain multiplex staining registration serial sections.Ĭopyright © 2021 Chen, Liu, Li, Gong, Long and Li.Ĭ-Fos activation in young and aging mouse brains in response to forced swimming stress. By removing the problems resulting from repeated manual operations, this pipeline is widely applicable to serial brain slices from multiple samples in a rapid and convenient manner, which benefits to facilitate research in life sciences. ![]() ![]() Finally, we validated the pipeline with immunostaining by analyzing the activity variance in the whole brain during acute stress in aging and young mice. Subsequently, using the self-developed analysis software, we registered and quantified the signals of imaged sections to the Allen Mouse Brain Common Coordinate Framework, which is compatible with multimodal images and slant section planes. First, we developed a serial brain-slice-staining method to stain serial sections and obtained more than 98.5% of slices with high integrity. In this study, we introduced a pipeline to acquire anatomical and histological information quickly and efficiently from serial sections. Previous studies have focused on several regions and have had difficulty analyzing serial tissue samples. Understanding the organization of different cells in the whole brain is crucial for investigating brain functions. The brain modulates specific functions in its various regions. ![]()
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